TRANSCRIPTION FACTORS AND miRNAS
We have screened a few thousand genes in primary neurons using overexpression strategies. The ones that seem the most effective at inducing axon growth in downstream validation assays and in vivo are genes that regulate many other targets. Genes for cytoskeletal elements, even though they might be very active in vitro, such as doublecortin, don’t do much in vivo. Consequently, we currently focus on kinases, transcription factors (like STAT3, KLF4 and KLF7, Jun) and miRNAs.
Lerch, J, Martinez, Y. Bixby, JL. Lemmon VP, cJun promotes CNS axon growth. Mol Cell. Neuroscience 2014 59:97-105.
Murray G. Blackmore, Zimei Wang, Jessica K. Lerch, Dario Motti, Yi Ping Zhang, Christopher B. Shields, Jae K. Lee, Jeffrey L. Goldberg, Vance P. Lemmon, and John L. Bixby. Kruppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract. Proc. Nat. Acad. Sci. 2012 109:7517-22.
Smith RP, Lerch-Haner JK, Pardinas JR, Buchser WJ, Bixby JL, Lemmon VP. Transcriptional profiling of intrinsic PNS factors in the postnatal mouse. Mol Cell Neurosci. 2011 46:32-44.
Murray G. Blackmore, Darcie L. Moore, Robin P. Smith, Jeffrey L. Goldberg, John L. Bixby, Vance P. Lemmon. High content screening of cortical neurons identifies novel regulators of axon growth. Molecular and Cellular Neuroscience. 2010 44:43-54.
Moore D, Blackmore M, Hu Y, Klaus H Kaestner, Bixby JL, Lemmon VP, Goldberg J. KLF Family Members Regulate Intrinsic Axon Regeneration Ability. Science. 2009 326:298-301
Lerch, J, Martinez, Y. Bixby, JL. Lemmon VP, cJun promotes CNS axon growth. Mol Cell. Neuroscience 2014 59:97-105.
Murray G. Blackmore, Zimei Wang, Jessica K. Lerch, Dario Motti, Yi Ping Zhang, Christopher B. Shields, Jae K. Lee, Jeffrey L. Goldberg, Vance P. Lemmon, and John L. Bixby. Kruppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract. Proc. Nat. Acad. Sci. 2012 109:7517-22.
Smith RP, Lerch-Haner JK, Pardinas JR, Buchser WJ, Bixby JL, Lemmon VP. Transcriptional profiling of intrinsic PNS factors in the postnatal mouse. Mol Cell Neurosci. 2011 46:32-44.
Murray G. Blackmore, Darcie L. Moore, Robin P. Smith, Jeffrey L. Goldberg, John L. Bixby, Vance P. Lemmon. High content screening of cortical neurons identifies novel regulators of axon growth. Molecular and Cellular Neuroscience. 2010 44:43-54.
Moore D, Blackmore M, Hu Y, Klaus H Kaestner, Bixby JL, Lemmon VP, Goldberg J. KLF Family Members Regulate Intrinsic Axon Regeneration Ability. Science. 2009 326:298-301
| molecular_and_cellular_neuroscience_2013_lerch.pdf |
| proceedings_of_the_national_academy_of_sciences_2012_blackmore.pdf |
| molecular_and_cellular_neuroscience_2011_smith.pdf |
| science_2009_moore.pdf |
| molecular_and_cellular_neuroscience_2010_blackmore-2.pdf |