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    • Macarena Vergara
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      • Sam Beckerman, MD/PhD student
      • Julia Brzac
      • Willi Buchser, PhD
      • Nathaniel Cleri
      • Danny Cooper, Ph.D.
      • Danny Cabrera
      • Pingping Chen, MD/PhD, Viral Vector Core
      • Matt Danzi, PhD student
      • Ellie Dawson
      • Roberto E Diaz
      • Kireeti Dulla
      • Laetitia Francelle, Ph.D.
      • Nathan Fox
      • Jared Gans, M.D.
      • Juliana Hemela
      • Pingping Jai, M.S. Viral Vector Core
      • Victor Jann
      • Danny L. John, MS1
      • Ryohei Kurosawa
      • Lyndah Lovell
      • Jeffrey Lowell
      • Nick O'Neill, PhD student
      • Kim Madhwani
      • Kar Men Mah, Ph.D.
      • Dario Motti, PhD
      • Melissa Munoz
      • Maya Murry: Computer Science REU
      • Michael Ortega
      • Marcos Rivas: HHMI SURF
      • Saloni Mehta, PhD student
      • Sevika Singh
      • Tatiana Slepak, M.S.
      • Robin Smith, PhD
      • Moya Tomlin, MS2
      • Enjolie Vadella
      • Eli Weaver, Lab Manager
      • Giulia Zunino, Ph.D.
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Light sheet fluorescence microscopy
We always want to go faster - we just got a new LSFM from 3i.  one of the first image sets was 2TB.   Those poor IT guys.

Picture
Picture
Brain preparation: Kar Men Mah and Abdiel Badillo-Martinez.  iDisco by Sensei Pantelis Tsoulfas.  Imaging and video by Ed Lachica at 3I
Pantelis Tsoulfas, Jae Lee, Kevin Park and their lab members do most of the work.  Vance and John cheer.  
3D Visualization of Individual Regenerating Retinal Ganglion Cell Axons Reveals Surprisingly Complex Growth Paths.  Bray ER, Noga M, Thakor K, Wang Y, Lemmon VP, Park KK, Tsoulfas P. eNeuro. 2017 Aug 29;4(4). pii: ENEURO.0093-17.2017. doi: 10.1523/ENEURO.0093-17.2017. eCollection 2017 Jul-Aug. PMID: 28856242

​Luo X, Salgueiro Y, Beckerman SR, Lemmon VP, Tsoulfas P, Park KK.  Three-dimensional evaluation of retinal ganglion cell axon regeneration and pathfinding in whole mouse tissue after injury.  Exp Neurol. 2013 Sep;247:653-62. 

Soderblom C, Luo X, Blumenthal E, Bray E, Lyapichev K, Ramos J, Krishnan V, Lai-Hsu C, Park KK, Tsoulfas P, Lee JK. Perivascular fibroblasts form the fibrotic scar after contusive spinal cord injury. J Neurosci. 2013 Aug 21;33(34):13882-7.

Soderblom C, Lee D, Dawood A, Carballosa M, Santamaria AJ, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Kevin K. Park KK, Guest JD, Lemmon VP, Jae K. Lee JK, Tsoulfas P 3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates  eNeuro March/April 2015, 2(2) e0001-15.2015 1–24


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  • Research
    • LemBix News
    • High Content Screening
    • Kinases and regeneration
    • PNS vs CNS Regeneration
    • RNA-Seq
    • Master Nodes
    • Light Sheet Fluorescent Microscopy
    • MIASCI: a reporting standard
    • RegenBase
    • Assay Guidance Manual
    • BioAssay Ontology
  • Bixby
    • John Bixby PubMed Serch
  • Lemmon
    • BLM
    • Xiao-Ming Xu
    • Miami Project SCI Course
    • Vance's Papers
    • Back in the day
    • SBI2
    • Growth Cone Videos
    • Bike Safety
    • Miscellaneous
  • Ali
  • Lab Members
    • Group Photos >
      • PAMM 160728
      • ProjectRock 190524
      • Escape Room 210524
    • Abdiel Badillo-Martinez
    • Yania Martinez, VVC Core >
      • Yania's Monarch
    • Yan Shi, M.S. Imaging and HCA Core
    • Macarena Vergara
    • Former Lab Members >
      • Sam Beckerman, MD/PhD student
      • Julia Brzac
      • Willi Buchser, PhD
      • Nathaniel Cleri
      • Danny Cooper, Ph.D.
      • Danny Cabrera
      • Pingping Chen, MD/PhD, Viral Vector Core
      • Matt Danzi, PhD student
      • Ellie Dawson
      • Roberto E Diaz
      • Kireeti Dulla
      • Laetitia Francelle, Ph.D.
      • Nathan Fox
      • Jared Gans, M.D.
      • Juliana Hemela
      • Pingping Jai, M.S. Viral Vector Core
      • Victor Jann
      • Danny L. John, MS1
      • Ryohei Kurosawa
      • Lyndah Lovell
      • Jeffrey Lowell
      • Nick O'Neill, PhD student
      • Kim Madhwani
      • Kar Men Mah, Ph.D.
      • Dario Motti, PhD
      • Melissa Munoz
      • Maya Murry: Computer Science REU
      • Michael Ortega
      • Marcos Rivas: HHMI SURF
      • Saloni Mehta, PhD student
      • Sevika Singh
      • Tatiana Slepak, M.S.
      • Robin Smith, PhD
      • Moya Tomlin, MS2
      • Enjolie Vadella
      • Eli Weaver, Lab Manager
      • Giulia Zunino, Ph.D.
  • Resources
    • Forms