LemBix Lab Techniques
Molecular Biology: cDNA library construction, Real-time PCR, RNA-seq, microarrays, knock-in mouse and transgenic mouse generation, mouse genetics, gene mapping
Microscopy: time-lapse, FRET, TIRF, High Content Screening, light sheet fluorescence microscopy
Cell Culture: primary culture of neurons (cortical, hippocampal, cerebellar granule neurons, DRGs, spinal cord), hybridoma production, transfection of primary neurons, culture of primary astrocytes
Viral Vectors: lenti, AAV
Biochemistry: HPLC, affinity purifications, western blotting, immunoprecipitations
Microscopy: time-lapse, FRET, TIRF, High Content Screening, light sheet fluorescence microscopy
Cell Culture: primary culture of neurons (cortical, hippocampal, cerebellar granule neurons, DRGs, spinal cord), hybridoma production, transfection of primary neurons, culture of primary astrocytes
Viral Vectors: lenti, AAV
Biochemistry: HPLC, affinity purifications, western blotting, immunoprecipitations
